The LL-100 panel

100 cell lines for blood cancer studies

The RNA-seq data from the LL-100 panel (ArrayExpress; ENA) allow expression analysis for single genes (bar chart) or for sets of genes (bar chart, heat map). Different to the description of the cited paper below, RNA-seq data were quantified via Salmon and normalised via DESeq2.

Bar chart Heat map Help

Hilmar Quentmeier, Claudia Pommerenke, Wilhelm G. Dirks, Sonja Eberth, Max Koeppel, Roderick A. F. MacLeod, Stefan Nagel, Klaus Steube, Cord C. Uphoff & Hans G. Drexler

For many years, immortalized cell lines have been used as model systems for cancer research. Cell line panels were established for basic research and drug development, but did not cover the full spectrum of leukemia and lymphoma. Therefore, we now developed a novel panel (LL-100), 100 cell lines covering 22 entities of human leukemia and lymphoma including T-cell, B-cell and myeloid malignancies. Importantly, all cell lines are unequivocally authenticated and assigned to the correct tissue. Cell line samples were proven to be free of mycoplasma and non-inherent virus contamination. Whole exome sequencing and RNA-sequencing of the 100 cell lines were conducted with a uniform methodology to complement existing data on these publicly available cell lines. We show that such comprehensive sequencing data can be used to find lymphoma-subtype-characteristic copy number aberrations, mRNA isoforms, transcription factor activities and expression patterns of NKL homeobox genes. These exemplary studies confirm that the novel LL-100 panel will be useful for understanding the function of oncogenes and tumour suppressor genes and to develop targeted therapies.

Read more in Scientific reports
Disease Abbreviation
Acute myeloid leukemia, erythroid AML_ery
Acute myeloid leukemia, megakaryocytic AML_mega
Acute myeloid leukemia, monocytic AML_mono
Acute myeloid leukemia, myelocytic AML_myelo
Anaplastic large cell lymphoma ALCL
B-cell precursor / pre-B-acute lymphoblastic leukemia pre-B-ALL
Burkitt lymphoma / B-acute lymphoblastic leukemia BL_B-ALL
Chronic lymphocytic leukemia / prolymphocytic leukemia CLL_PLL
Chronic myeloid leukemia, lymphoid blast crisis CML_lympho_BC
Chronic myeloid leukemia, myeloid blast crisis CML_myelo_BC
Diffuse large B-cell lymphoma, activated B-cell DLBCL_ABC
Diffuse large B-cell lymphoma, germinal center B-cell DLBCL_GCB
Hairy cell leukemia HCL
Hodgkin lymphoma HL
Mantle cell lymphoma MCL
Mature T-malignancy mature_T
Multiple myeloma, plasma cell leukemia MM_PCL
Myeloproliferative neoplasm MPN
Natural killer malignancy NK
Primary effusion lymphoma PEL
Primary mediastinal B-cell lymphoma PMBL
T-acute lymphoblastic leukemia / T-lymphoblastic lymphoma T-ALL_TLL