Cell line: MB-04
ACC-No.: 122
Date of analysis: 13.10.2014
DNA processing number:
Methode: DNA Barcoding by PCR amplification of 5´coding region of cytochrome c oxidase I (658 bp fragment size). Cycle sequencing of respective PCR products revealed following assignment upon submission to BOLD (Ratnasingham, S., Hebert, P. D. N. (2007) BOLD: The Barcode of Life Data System (www.Barcodinglife.org). Molecular Ecology Notes, 2007; 7(3): 355-364
Primer:
1x LepF1_t1: ATT TAG GTG ACA CTA TAG ATT CAA CCA ATC ATA AAG ATA TTG G
1x VF1_t1: ATT TAG GTG ACA CTA TAG TCT CAA CCA ACC ACA AAG ACA TTG G
1x VF1d_t1: ATT TAG GTG ACA CTA TAG TCT CAA CCA ACC ACA ARG AYA TYG G
3x VF1i_t1: ATT TAG GTG ACA CTA TAG TCT CAA CCA ACC ANA ANG ANA TNG G
1x LepR1_t1: TAA TAC GAC TCA CTA TAG GGT AAA CTT CTG GAT GTC CAA AAA ATC A
1x VR1d_t1: TAA TAC GAC TCA CTA TAG GGT AGA CTT CTG GGT GGC CRA ARA AYC A
1x VR1_t1: TAA TAC GAC TCA CTA TAG GGT AGA CTT CTG GGT GGC CAA AGA ATC A
3x VR1i_t1: TAA TAC GAC TCA CTA TAG GGT AGA CTT CTG GGT GNC CNA ANA ANC A
Sequence:
5`-TWWTTTTWRRGSGACACTATAGWYTCAACCAAYCAYAAAGAYATTGGTACATTATATTTT
ATTTTTGGGATTTGAGCAGGAATAGTAGGAACTTCACTAAGTTTATTAATTCGAGCTGAA
TTAGGAAACCCTGGATCTTTAATTGGAGATGATCAAATTTATAATACTATTGTTACAGCT
CATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGTAAT
TGACTCGTTCCATTAATGTTAGGGGCCCCTGATATAGCATTCCCTCGAATAAATAATATA
AGTTTTTGACTTCTTCCCCCTTCTTTAACTCTTTTAATTTCAAGTAGAATCGTAGAAAAT
GGAGCAGGAACAGGATGAACAGTGTACCCCCCACTTTCATCTAATATTGCCCATGGAGGA
AGTTCAGTAGATTTAGCTATTTTTTCACTTCATTTAGCTGGTATTTCTTCAATTCTCGGA
GCTATTAATTTTATTACTACAATTATTAATATACGATTAAATAGTTTATCTTTTGATCAA
ATACCTTTATTTATTTGAGCTGTAGGAATTACTGCATTTTTATTATTATTATCTTTACCT
GTATTAGCTGGAGCTATTACTATACTTTTAACAGATCGAAATTTAAATACATCTTTTTTT
GACCCTGCRGGRGGRGKTGWYCCAAWTTTWWTAYCAAMMWTTWATTKKTATTTYTKGACW CT-3`
| Taxonomic Level | Taxon Assignment | Probability of Placement (%) |
|---|---|---|
| Phylum | Arthropoda | 100 |
| Class | Insecta | 100 |
| Order | Lepidoptera | 100 |
| Family | Noctuidae | 100 |
| Genus | Mamestra | 100 |
| Species | Mamestra brassicae | 100 |
Identification Summary:
Search Result: The submitted sequence has been matched to Mamestra brassicae. This identification is solid unless there is a very closely allied congeneric species that has not yet been analyzed. Such cases are rare.