Cell line: BTI-EAA
ACC-No.: 176
Date of analysis: 21.11.2014
DNA processing number: 10053
Methode: DNA Barcoding by PCR amplification of 5´coding region of cytochrome c oxidase I (658 bp fragment size). Cycle sequencing of respective PCR products revealed following assignment upon submission to BOLD (Ratnasingham, S., Hebert, P. D. N. (2007) BOLD: The Barcode of Life Data System (www.Barcodinglife.org). Molecular Ecology Notes, 2007; 7(3): 355-364
Primer:
1x LepF1_t1: ATT TAG GTG ACA CTA TAG ATT CAA CCA ATC ATA AAG ATA TTG G
1x VF1_t1: ATT TAG GTG ACA CTA TAG TCT CAA CCA ACC ACA AAG ACA TTG G
1x VF1d_t1: ATT TAG GTG ACA CTA TAG TCT CAA CCA ACC ACA ARG AYA TYG G
3x VF1i_t1: ATT TAG GTG ACA CTA TAG TCT CAA CCA ACC ANA ANG ANA TNG G
1x LepR1_t1: TAA TAC GAC TCA CTA TAG GGT AAA CTT CTG GAT GTC CAA AAA ATC A
1x VR1d_t1: TAA TAC GAC TCA CTA TAG GGT AGA CTT CTG GGT GGC CRA ARA AYC A
1x VR1_t1: TAA TAC GAC TCA CTA TAG GGT AGA CTT CTG GGT GGC CAA AGA ATC A
3x VR1i_t1: TAA TAC GAC TCA CTA TAG GGT AGA CTT CTG GGT GNC CNA ANA ANC A
Sequence:
5`-CAYAAAGYTTGGACATTATATTTTATTTTTGGARTKMRAGCAGGAATAGTTGGAACATCA
CTAAGATTATTAATTCGTGCAGAATTGGGTAATCCTGGGTCTTTAATTGGAGATGATCAA
ATTTATAACACTATTGTAACAGCTCATGCTTTTATTATAATTTTTTTTATAGTTATGCCA
ATTATAATTGGAGGATTTGGAAATTGATTAGTTCCTTTAATATTAGGAGCTCCTGATATA
GCTTTTCCACGAATAAATAATATAAGTTTTTGACTTTTACCCCCATCATTAACTTTATTA
ATTTCAAGAAGAATTGTAGAGAATGGAGCAGGAACTGGATGAACAGTATATCCCCCCCTT
TCTTCTAATATTGCTCATGGGGGAAGATCCGTTGATTTAGCTWTTTTTYCCYTYMWTTWR
CMRGGAWTTYYTYMAWTTTARGAACCATTAATTTTATTACTACCATTTTTAATWTWCCAA
TAAATAATTTAACAWTTGAACAAATWCCTTTATTTGGTTGAACCGGWRGAATTACCGGTT
TTTTAATACTTTYTTCCCTTCCYGGTTTAACMRGGRCCATTWCTATWTTACTAACMRAAC
SRAATTTAAATWCCTYAWTTTTTGACCCMRCGGGRGGAAGGAAACCYATTCYTTAAYAAC
MCTTATTTTGATTTTTTGGRCCWCCMRAARTTTACCCTATWRTGAGTCSKAATTAA-3`
NCBI blast
ORGANISM: Estigmene acrea
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
Pterygota; Neoptera; Endopterygota; Lepidoptera; Glossata;
Ditrysia; Noctuoidea; Arctiidae; Estigmene.
Search Result BOLD System: no match
Catalog: Estigmene acrea