Cell line: MCA-B1
ACC-No.: 828
Lot-No.: -
Date of analysis: 11.07.2018
DNA processing number: 14760
Methode: DNA Barcoding by PCR amplification of 5´coding region of cytochrome c oxidase I (658 bp fragment size). Cycle sequencing of respective PCR products revealed following assignment upon submission to BOLD (Ratnasingham, S., Hebert, P. D. N. (2007) BOLD: The Barcode of Life Data System (www.Barcodinglife.org). Molecular Ecology Notes, 2007; 7(3): 355-364
Primer:
1x VF1d_t1: ATT TAG GTG ACA CTA TAG TCT CAA CCA ACC ACA ARG AYA TYG G
1x VR1_t1: TAA TAC GAC TCA CTA TAG GGT AGA CTT CTG GGT GGC CAA AGA ATC A
Sequence:
5`-CTCACCACTAGCATATCGGTACTTTATACTTACTATTTGGAGCATGAGCCGGTATAGTAG
GCACTGCCTTGAGCCTCCTCATCCGAGCCGAACTAGGTCAGCCCGGTACTTTACTAGGTG
ACGATCAAATTTATAATGTCATCGTAACCGCCCATGCTTTCGTAATAATCTTCTTCATAG
TCATGCCCATCATAATTGGGGGCTTTGGAAACTGACTAGTGCCGTTAATAATTGGTGCTC
CGGACATGGCATTCCCCCGAATAAATAACATGAGCTTCTGACTCCTTCCTCCATCCTTTC
TTCTACTATTAGCATCTTCTATGGTAGAAGCAGGTGCAGGAACGGGATGAACCGTATACC
CCCCACTGGCTGGCAATCTGGCCCATGCAGGAGCATCCGTTGACCTTACAATTTTCTCCT
TACACTTAGCCGGAGTCTCTTCTATTTTAGGGGCAATTAATTTCATCACTACTATTATCA
ACATAAAACCCCCTGCAATATCCCAGTATCAAACTCCCCTGTTTGTATGATCAGTACTAA
TTACAGCAGTTCTACTCTTACTATCCCTGCCTGTACTGGCTGCTGGAATTACAATACTTT
TAACAGACCGGAATCTTAATACAACATTTTTTGATCCCGCTGGAGGAGGAGACCCTATCC
TATATCACCACCTATTCTGATTCTCGGCCAAACCAGAGNTCTACCCTATAGTGAGTCGAT
TTAAAAA-3`
| Taxonomic Level | Taxon Assignment | Probability of Placement (%) |
|---|---|---|
| Phylum | Chordata | 100 |
| Class | Mammalia | 100 |
| Order | Carnivora | 100 |
| Family | Canidae | 100 |
| Genus | Canis | 100 |
Identification Summary:
Search Result: A species level match could not be made, the queried specimen is likely to be one of the following:
Canis lupus
Canis lupus familiaris
Canis familiaris
Canis lupus pallipes
