Cell line: A-2

Cell line: A-2

ACC-No.: 276

Date of analysis: 13.10.2014

DNA processing number:

Methode: DNA Barcoding by PCR amplification of 5´coding region of cytochrome c oxidase I (658 bp fragment size). Cycle sequencing of respective PCR products revealed following assignment upon submission to BOLD (Ratnasingham, S., Hebert, P. D. N. (2007) BOLD: The Barcode of Life Data System (www.Barcodinglife.org). Molecular Ecology Notes, 2007; 7(3): 355-364

Primer:

1x LepF1_t1: ATT TAG GTG ACA CTA TAG ATT CAA CCA ATC ATA AAG ATA TTG G

1x VF1_t1: ATT TAG GTG ACA CTA TAG TCT CAA CCA ACC ACA AAG ACA TTG G

1x VF1d_t1: ATT TAG GTG ACA CTA TAG TCT CAA CCA ACC ACA ARG AYA TYG G

3x VF1i_t1: ATT TAG GTG ACA CTA TAG TCT CAA CCA ACC ANA ANG ANA TNG G

1x LepR1_t1: TAA TAC GAC TCA CTA TAG GGT AAA CTT CTG GAT GTC CAA AAA ATC A

1x VR1d_t1: TAA TAC GAC TCA CTA TAG GGT AGA CTT CTG GGT GGC CRA ARA AYC A

1x VR1_t1: TAA TAC GAC TCA CTA TAG GGT AGA CTT CTG GGT GGC CAA AGA ATC A

3x VR1i_t1: TAA TAC GAC TCA CTA TAG GGT AGA CTT CTG GGT GNC CNA ANA ANC A

Sequence:

5`-CACGAAGMTTGGCACCCTTTATCTAGTATTTGGCGYMMGGGCCGGTATGGTGGGGACAGC

CTTAAGCCTCTTAATTCGAGCCGAACTAAGTCAACCTGGCACCCTCCTGGGTGACGACCA

AATCTACAATGTGATCGTCACAGCTCATGCCTTTGTAATAATTTTTTTTATAGTCATACC

AATCATAATTGGCGGCTTTGGTAACTGATTGATCCCACTAATAATCGGCGCTCCCGACAT

AGCCTTCCCCCGAATAAATAACATAAGCTTTTGACTCCTTCCCCCCTCATTTCTTCTTCT

TCTAGCATCCTCCGGAGTTGAAGCAGGGGCTGGAACTGGGTGAACTGTTTATCCCCCTCT

TGCAGGTAATTTGGCACATGCTGGGCCCTCCGTGGACTTGACTATTTTTTTCACTCCACT

TGGCTGGKGTTTCCTCCATTTTAGGGGCAATCAACTTTWWTCACCMCAWTAATTAACATA

AAACCCCCCSSCAGCATYTCAWWMCCMRRAMACCCCTGKTTTKKYYTRRSCCKTYYTAAT

TACCRSCCGTWMYTYCYWMYTTYTTTTYCCYCCCCSKYCYTKSCCSMRGKWTTACCMAGY

YTTTTACMRAWYSRAAAWYYTWAMMCCCMCYTTTTTTRMCCCSMRGKGGGGGRRAAMCCA

WWYYTTTMMMAAMMYTTTTYKRKWTTTTKGGCCCCCCMRARWTTMCCCYTTKKGGWGGSK

TWAAAA-3`

Identification Summary:

Search Result: A species level match could not be made, the queried specimen is likely to be one of the following:

Xiphophorus hellerii

Poecilia latipinna

Xiphophorus variatus