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RBL-2H3

DSMZ: ACC-312

Species: Rattus norvegicus (rat)

Cell type: basophilic leukemia

Incubation medium: 70% MEM (with Earle's salts) + 20% RPMI 1640 + 10% h.i. FBS; a higher proportion of RPMI 1640 may increase proliferation rate, but decreases histamine production

Morphology: adherent, fibroblast-like cells growing mainly as monolayer and in a small extend as suspension culture

COI DNA Barcoding Report

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Cell line: RBL-2H3

ACC-No.: 312

Date of analysis: 13.10.2014

DNA processing number:

Methode: DNA Barcoding by PCR amplification of 5┬┤coding region of cytochrome c oxidase I (658 bp fragment size). Cycle sequencing of respective PCR products revealed following assignment upon submission to BOLD (Ratnasingham, S., Hebert, P. D. N. (2007) BOLD: The Barcode of Life Data System (www.Barcodinglife.org). Molecular Ecology Notes, 2007; 7(3): 355-364

Primer:

1x LepF1_t1: ATT TAG GTG ACA CTA TAG ATT CAA CCA ATC ATA AAG ATA TTG G

1x VF1_t1: ATT TAG GTG ACA CTA TAG TCT CAA CCA ACC ACA AAG ACA TTG G

1x VF1d_t1: ATT TAG GTG ACA CTA TAG TCT CAA CCA ACC ACA ARG AYA TYG G

3x VF1i_t1: ATT TAG GTG ACA CTA TAG TCT CAA CCA ACC ANA ANG ANA TNG G

1x LepR1_t1: TAA TAC GAC TCA CTA TAG GGT AAA CTT CTG GAT GTC CAA AAA ATC A

1x VR1d_t1: TAA TAC GAC TCA CTA TAG GGT AGA CTT CTG GGT GGC CRA ARA AYC A

1x VR1_t1: TAA TAC GAC TCA CTA TAG GGT AGA CTT CTG GGT GGC CAA AGA ATC A

3x VR1i_t1: TAA TAC GAC TCA CTA TAG GGT AGA CTT CTG GGT GNC CNA ANA ANC A

Sequence:

5`-WWTTTWAGTACACTATAGTCTCARSCAACCACAARGACATTGGAACCCTCTACCTATTAT

TTGGAGCCTGAGCAGGAATAGTAGGGACAGCTTTAAGTATTCTAATTCGAGCTGAACTAG

GACAGCCAGGCGCACTCCTAGGAGATGACCAAATCTATAATGTCATCGTCACAGCCCATG

CATTCGTAATAATTTTCTTTATAGTAATACCTATAATAATTGGAGGCTTCGGGAACTGAC

TTGTACCACTAATAATTGGAGCCCCTGATATAGCATTCCCACGAATAAATAACATAAGCT

TTTGACTGCTTCCTCCATCATTTCTACTCCTTTTAGCATCCTCCATAGTAGAAGCTGGAG

CTGGAACAGGATGAACAGTATACCCCCCCTTAGCCGGAAACCTAGCCCATGCTGGAGCAT

CCGTAGATTTAACTATTTTTTCCCTCCACCTAGCCGGGGTGTCTTCTATCTTAGGAGCTA

TCAACTTTATCACCACTATCATTAATATAAAACCCCCTGCTATAACCCAATATCAGACAC

CTCTCTTTGTATGATCCGTACTAATTACAGCCGTCCTACTACTTCTCTCACTGCCAGTAT

TAGCAGCAGGTATCACTATACTCCTTACAGACCGAAATCTAAATACTACTTTCTTCGACC

CCGCTGGAGGTGGRGAMCCMAWTCYYYAWYMAMCAMCYATWTCKRTACTCTTGCCACCT-3`

Taxonomic Level Taxon Assignment Probability of Placement (%)
Phylum Chordata 100
Class Mammalia 100
Order Rodentia 100
Family Muridae 100
Genus Rattus 100

Identification Summary:

Search Result: A species level match could not be made, the queried specimen is likely to be one of the following:

Rattus rattus

Rattus norvegicus

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